摘要
研究了猪血中SOD的提取工艺,对影响SOD活性的破碎条件和热变性条件进行了探讨.结果显示：（1）等体积水稀释红细胞,经200 w1、5 min超声波破碎后SOD总活性比机械搅拌高出1.2倍.（2）热变性时加入1.0 mL 0.3 mol.L^-1CuCl2/50 mL红细胞,55-65℃热变性15 min,有效的提高了SOD比活.经检测SOD总活性为593 965.2 U.L^-1红细胞,SOD活性提取率为83.3%,比活为1 650.9 U.mg^-1.
Distilled water was added into red blood cells according to the volume proportion of 1 ： 1, and were disrupted by ultrasonic for 15 min at 200 W. The total activity is 1.2 times than mechanical agitation. In the process of heat modification about 1.0 mL 0.3 mol·L^-1 CuCl2 was added in 50 mL red blood cell, and heat preservation at 55-65℃ for 15 min. The total activity of SOD was 593 965.2 U· L^-1 red blood cell ,and extraction rate was 83.3% and the specific activity was 1 650.9 U · mg^-1.
出处
《陕西科技大学学报（自然科学版）》
2007年第3期50-52,共3页
Journal of Shaanxi University of Science & Technology
基金
陕西省教育厅专项项目资助
项目编号:JK05-145
关键词
SOD
热变性
提取率
比活
SOD
heat modification
extraction rate
specific activity
