低浓度哇巴因对白血病细胞生长的影响

Effects of Ouabain at Low Concentrations on Growth of Leukemia Cells

本研究目的在于通过研究低浓度哇巴因对不同白血病细胞株生长的影响,探讨哇巴因用于白血病临床治疗的可行性。采用MTT法和流式细胞术检测低浓度(≤10nmol/L)哇巴因对白血病细胞株生长的作用及细胞周期的影响,Western blot检测各细胞株钠泵α1亚单位在蛋白水平上的表达及哇巴因作用下的变化情况。结果表明:低浓度哇巴因可抑制巨核系白血病M07e、Meg-01细胞株的增殖,且呈浓度依赖性;10nmol/L哇巴因作用24小时,这类细胞株的细胞周期被阻滞于G1期;其钠泵α1亚单位基础表达较低,经哇巴因干预后表达水平进一步降低;在相同浓度哇巴因作用下,淋巴系白血病B95、Jhhan细胞株出现增殖,S期及G2/M期细胞比率增高,其钠泵α1亚单位的表达也增高。结论:低浓度哇巴因可抑制巨核系白血病细胞株M07e、Meg-01的生长,这可能与该细胞株钠泵α1亚单位低表达及哇巴因对其的负调控有关。

This study was aimed to investigate the effects of ouabain at low concentrations on growth regulation in various leukemia cell lines and to determine the therapeutic potential of ouabain in leukemia. By using MTT, flow cytometry（ FCM）, the changes in cell growth and cell cycle of leukemia cell lines were observed after treating with ouabain at low concentrations（ ≤ 10 nmoL/L）. The expression of sodium pump α1 subunit was evaluated by Western blot. The results showed that in megakaryocytic leukemia M07e and Meg-01 cell lines, the low concentrations of ouabain （ ≤ 10 nmoL/L） inhibited the cell growth, blocked the cell cycle at G1 phase, and decreased the protein expression of sodium pump α1 subunit. The same concentrations of ouabain induced the proliferation of lymphocytic leukemia B95 and Jhhan cell lines, increased the percentage of cells at S phase and G2/M phase and up-regulated the expression of sodium pump α1 subunit. The basic expression of the sodium pump α1 subunit in M07e and Meg-01 was lower than that in B95 and Jhban. R is concluded that the low concentrations of ouabain inhibit the cell growth of the megakaryocytic leukemia cell lines M07e and Meg-0, which may be related with the low expression of sodium pump α1 subunit of that cell lines and the negative regulation of the protein induced by ouabain.