口腔鳞癌中骨桥蛋白的表达及意义

Quantitative analysis and significance of osteopontin expression in oral squamous cell carcinoma

目的:探讨口腔鳞癌(OSCC)组织中骨桥蛋白(osteopontin,OPN)的表达及其临床意义。方法:应用实时荧光定量PCR法(FQ-PCR)检测OSCC肿瘤组织和癌旁对照组织(n=16)中骨桥蛋白mRNA的表达;应用EnVisionTM法检测正常口腔黏膜(n=12)和OSCC患者肿瘤组织(n=59)中骨桥蛋白的表达,结合图像分析系统进行定量分析,应用SPSS11.5软件包进一步分析不同临床、病理指标下OSCC肿瘤组织骨桥蛋白的表达情况。结果:16例患者OPN在OSCC肿瘤组织中的表达,mRNA和蛋白水平均显著高于正常口腔黏膜(P<0.05)。59例蜡块标本的OPN表达在OSCC不同临床分期间、有无颈淋巴结转移间均有显著差异,而在OSCC高分化和中低分化者间无显著差异。结论:在OSCC中,OPN无论是分子还是蛋白水平,均存在过度表达,其表达水平与肿瘤临床分期以及有无淋巴结转移存在一定的相关性。

PURPOSE： To study the clinical significance of osteopontin （OPN） expression in oral squamous cell carcinoma （OSCC）, METHODS： OPN expression was examined in OSCC and normal oral mucosa （NOM） by the method of En VisionTM and fluorescence quantitative real-time PCR, the staining-grade was quantitatively studied by multiple functional image analyzer. OPN expression grade of different clinical and pathological index was statistically evaluated using SPSS11.5 software package. RESULTS： OPN expression grade was significantly higher in OSCC than that in NOM in both the mRNA level and protein level（P〈0.05）, significant difference of OPN expression grade was found between the different clinical stages, as well as between the groups with or without cervical lymph node metastasis. The group with cervical lymph node metastasis （n=20） had higher expression than that of the group without lymph node metastasis（n=39, P〈0.05）.However, there was no significant difference between the expression grade in well-differentiated （n=38） group and moderate or poorly differentiated group （n=21）. CONCLUSION： OPN overexpression is found in OSCC , and the expression level correlates with the clinical staging and cervical lymph node metastasis.