PCR法检测淋球菌、沙眼衣原体及解脲支原体感染

DIAGNOSIS OF NEISSERIA GONORRHOEAE, CHLAMYDIA TRACHOMATIS AND UREAPLASMA UREALYTICUM INFECTIONS BY PCR

目的：评价聚合酶链反应（ＰＣＲ）技术对性病性尿道炎及宫颈炎诊断的临床实用价值。方法：采用ＰＣＲ法、淋球菌（Ｎｇ）涂片／培养、沙眼衣原体直接免疫荧光检查（ＣｔＤＩＦＡ）和解脲支原体（Ｕｕ）培养平行检测了１１９例患者的１８９份尿道／宫颈拭子标本。结果：以Ｎｇ涂片／培养、ＣｔＤＩＦＡ和Ｕｕ培养为对比标准，ＮｇＰＣＲ、ＣｔＰＣＲ和ＵｕＰＣＲ的敏感性分别为１００％（２８／２８）、９６９％（３１／３２）和９３３％（４２／４５），特异性分别为９４５％（８６／９１）、９１９％（８０／８７）和９４６％（７０／７４）。治疗结束后１周～２周内复查仍有２５９％（２１／７０）患者ＰＣＲ未阴转。结论：ＰＣＲ技术可以敏感、特异地检测泌尿生殖道标本中的ＮｇＤＮＡ、ＣｔＤＮＡ和ＵｕＤＮＡ，具有简便、快速的优点，然而对治疗后短期内复诊患者，似不宜仅以ＰＣＲ阳性结果作为重复治疗的依据。

Objective:To evaluate the clinical application of polymerase chain reaction(PCR)in diagnosing sexually transmitted urethritis and cervicitis.Method:189 urogenital specimens from 119 patients were analyzed in parallel by PCR,stained smears or culture method for Neisseria gonorrhoeae,direct immunofluorescence assay(DIFA) for Chlamydia trachomatis,culture for Ureaplasma urealyticum.Results:Compared with the Ng stained smears or culture,CtDIFA and Uu culture,the sensitivity of NgPCR,CtPCR,and UuPCR were 100%(28/28),96 3%(31/32) and 93 3%(42/45),the specificity were 94 5%(86/91),91 9%(80/87),and 94 6%(70/74),respectively.70 cases who were positive by PCR before treatment were followed up in one or two weeks after the completion of treatments.And the PCR of 25 9%(21/70)was still positive.Conclusions:PCR is a sensitive and specific procedure to detect NgDNA,CtDNA and UuDNA in urogenital specimens,and takes the advantage of being relative simple and time saving.However,for patients shortly after treatments, it seems less reliable to give repetitive medication depending only on positive results of PCR.