玉米分离群体家系籽粒赖氨酸含量测定适用方法的筛选

Screening of the Chemical Methods Applied to Lysine Content Determination of the Family Seeds from Segregating Population in Maize

以普通玉米和o2玉米为材料,对茚三酮显色法、染料结合赖氨酸(DBL)法、2-氯-3,5-二硝基吡啶(CDNP)法和2,4,6-三硝基苯磺酸(TNBS)法等4种化学分析方法,从标准曲线的回归方程、样品赖氨酸含量测定的结果、工作原理和实验操作等方面进行分析比较。结果表明,2-氯-3,5-二硝基吡啶法是一种相对准确和精确、操作较易、费用适中且耗时较少的赖氨酸含量分析方法,尤其适用于普通玉米和高赖氨酸玉米(特别是带有修饰基因者)组配的分离群体家系种子的赖氨酸含量分析。

Using normal maize and o2 maize as materials, the four colorimetric methods of ninhydrin, acid orange-12 dye binding lysine （DBL）, 2-Chloro-3,5-dinitropyridine （CDNP） and 2,4,6-Trinitrobenzene sulfonic acid （TNBS） were compared and discussed in the regression equation of calibration curve, the lysine content determined by means of these methods, the assay principles and the laboratorial work. The results showed that the colorimetry of 2-Chloro-3,5-dinitropyridine is relatively precise, easy operating, middle costing and less time consuming, especially applicable to the assay of lysine content of families in segregating population from a combination of normal type line and high-lysine line（particularly one with modifier genes）.