Yam-SF的免疫调节作用

Immnunoregulatory activity of Yam-SF

目的研究人B淋巴母细胞株Yam1B产生的免疫调节团子Yam－SF的免疫调节作用。方法取Yam1B细胞培养上清，于体外培养体系中观察其对人T细胞及B细胞增殖、免疫球蛋白产生、IL-2产生及IL-2表达的影响；测定分子量，并研究其理化性质；观察抗TGF－beta抗体对其生物活性的影响。结果Yam－SF可抑制人T细胞及B细胞的增殖，并抑制正常B细胞免疫球蛋白的产生，但对PHA刺激的人淋巴细胞兄-2的产生以及IL－2受体（IL－2R）的表达无抑制作用。Yam－SF经56℃处理10min或在pH10．0的环境中处理6h抑制活性丧失，Yam－SF经pronase水解消化而失活，在pH2．0的环境中保持其抑制活性。凝胶过滤实验表明，培养上清抑制活性分布在分子量43kD～67kD范围内，且抗TGF－beta抗体不能阻断其抑制活性。结论Yam－SF是一种与TGF—beta等免疫抑制因子不同的新的免疫调节团子。

To investigate the immunoroplatory activity of Yam-SF which was spontanansly pro-duced by Yam 1B, a huxnan B lymphoblastord .lineMethods To add Yam 1B culture supamatant into the human lymphocyte culture systern and check the proiferation of human T and B lymphocytes and aseay the immunoglobulin generation by normal B cells or IL -2 preduction and the mpression of 1L-2R. The physical and chemical characters and the effect of anti -TGF-beta antibody on the immunoregulatory ac-tivity of Yam-SF were investigted.Results Ywm-SF suppressed the proihation of human T and B lymphocytes and inhibites im-munoglobulin generation by normal B cells, but did not inhibit neither the expression of 1L-2 receptor nor the preduction of IL-2 in the lymphocytes stimulated with PHA. The inhibitory activity of Yam-SF was inactivated at 56℃ 10 min and at pH10. 0 for 6h and abrogated by digestion with pronase, but resistant to pH2. 0 a dministration. Gel-filtration column as-say showed a peak of inhibitory activity of cell proif-eration associated with molecules of apparent MW of 43kD to 67kD. The inLhibitory activity was notblocked by the anti-TGF-beta antidriy.Condusion The Yam-SF appeared to represent a nove immunoregulatory factor which differ frim TGF-beta and other irnrnne inhibited factors.