胆固醇诱导内皮细胞凋亡及抑制细胞增殖的实验研究

Human Umbilical Vein Endothelial Cell Apoptosis and Its Proliferative Inhibition Induced by Cholesterin

背景与目的:探讨胆固醇(cholesterin)对人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)凋亡及细胞增殖的影响,同时观察CuSO4溶液在其中所起的作用。材料与方法:实验分为8组:对照组(等体积溶剂)、胆固醇低剂量组(25mg/L)、中剂量组(50mg/L)和高剂量组(100mg/L);另外4组分别为以上各组加入终浓度为10μmol/L的CuSO4溶液。应用流式细胞仪检测不同浓度胆固醇对细胞凋亡率的影响;应用四甲基偶氮唑蓝微量酶反应比色法(MTT试验)检测细胞的生存率。结果:不同浓度的胆固醇可不同程度地诱导HUVEC细胞发生凋亡,可明显抑制HUVEC细胞增殖,且随浓度增高,其作用均明显增强。同时发现各组加入CuSO4溶液后可使其细胞凋亡率明显增加,生存率下降。结论:胆固醇可以诱导内皮细胞凋亡及抑制细胞增殖,CuSO4溶液可促进胆固醇对HUVEC的损伤作用。

BACKGROUND ＆ AIM： To study the effect of cholesterin on apoptosis and inhibition of proliferation in human umbilical vein endothelial ceils （HUVECs） and evaluate the role of CuSO4 in the process. MATERIALS AND METHODS： HUVECs were treated with different culture media： control（with equal volume of solvent）, lower-dose group （LD, 25 mg/L of cholesterin）, medium-dose group （MD, 50 mg/L of cholesterin）, high-dose group （HD, 100 mg/L of cholesterin）, control ＋ 10 μmol/L CuSO4 , LD ＋ 10 μmol/L CuSO4 , MD ＋ 10 μmol/L CuSO4 and HD ＋ 10 μmol/L CuSO4. Apoptosis in HUVECs were analyzed by flow cytometry and proliferation rate of HUVECs were determined by MTF test. RESULTS： Different doses of cholesterin caused different degrees of apoptosis and inhibition of proliferation in HUVECS. With increasing concentration of cholesterin , the effects on HUVECs were enhanced. At the same time, when CuSO4 was added, apoptosis and inhibition of proliferation in HUVECs became more severe. CONCLUSION： Cholesterin could induce apoptosis of HUVECs and inhibit proliferation of HUVECs. CuSO4 could accelerate the effect of eholesterin on HUVECs.