Cap43蛋白在人肺癌组织中的表达

Expression of Cap43 in Human Lung Cancer

背景与目的:探讨Cap43蛋白在肺癌组织中表达及其与组织学类型之间的关系。材料与方法:分别采用免疫组化法检测79例临床肺癌患者肿瘤组织和免疫印迹法检测28例临床肺癌患者新鲜肿瘤组织中Cap43蛋白的表达,并用图像分析系统进行半定量分析。结果:免疫组化结果显示,Cap43蛋白在细胞内分布以胞质为主,也有部分分布在胞核;其半定量结果显示,Cap43蛋白在非小细胞肺癌组织中表达量明显高于癌旁正常对照组织(P<0.05),且在肺腺癌组织中表达量明显高于肺鳞癌组织(P<0.01)。免疫印迹也显示,Cap43蛋白在非小细胞肺癌组织中高表达;其半定量结果显示,Cap43蛋白在肺粘液性腺癌组织中的表达量明显高于其它组织学分型肺癌组织(P<0.01)。随着肺癌组织分化程度的增高,Cap43蛋白表达量有明显降低的趋势(P<0.05)。两种检测方法均提示Cap43蛋白在癌旁正常对照组织和小细胞肺癌中低表达。免疫印迹检测方法所测阳性率(82.14%)高于免疫组化法(69.62%),但两种检测方法的阳性率间无明显差异(P>0.05)。结论:Cap43蛋白在非小细胞肺癌组织中高表达,该蛋白表达水平与肺癌组织学分型密切相关。

BACKGROUND ＆ AIM： To study the correlation between the level of Cap43 expression and histological pattem in hunman lung cancer. MATERIALS AND METHODS： The method of immunohistochemistry was used to detect Cap43 expression in 79 lung cancer specimens and western blot was used to detect its expression in 28 fresh lung cancer specimens and their surrounding normal controls.The results were semiquantitatively analyzed by image analysis systems. RESULTS： Immunohistochemistry staining revealed the distribution of Cap43 was mainly in cell cytoplasm. However,parts of the proteins were in the nucleus. Cap43 expression in lung cancer was obviously higher than normal controls （P 〈 0.05）. ＇It was also found that Cap43 expression in patient with adenocarcinoma was higher than those with squamous cell carcinoma（P〈0.01） Westem blot also showed high expression of cap43 in lung cancer. Cap43 expression in mucoid adenocarcinoma was significantly higher than the other pathological types of lung cancer. （P〈0.01） .It was shown that the less the lung cancer differentiated, the higher the Cap43 expression was（P〈 0.05）. Two detection methods together demonstrated Cap43 expression in normal controls and small cell lung cancers was very low. The positive rate of westem blot（82.14%） was higer than immunohistochemistry（69.62%）,but the two detection methods were not significantly different（P 〉 0.05） . CONCLUSION： Cap43 was highly expressed in non-small cell lung cancer and the level of expression was closely correlated with the histological pattern of tumor.