摘要
背景与目的:探讨一种简便的γ射线照射致小鼠衰老模型。材料与方法:将45只小鼠随机分成3组:对照组和2个模型组,对照组正常饲养,2个模型组分别给予不同剂量的γ射线照射。50d后观察血常规、胸腺指数、脾脏指数、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、脂褐素含量、β-半乳糖苷酶染色结果。结果:与对照组相比,2个模型组的血常规相关指标、胸腺指数和脾脏指数和SOD活性均降低(P<0.05);MDA含量和脂褐素含量升高(P<0.05);β-半乳糖苷酶染色呈阳性改变。结论:采用γ射线照射建立急性小鼠衰老模型的方法可行。
BACKGROUND ＆ AIM： To establish a convenient acute aging model by γ-ray irradiation. MATERIANLS： METHODS： 45 mice were divided into 3 groups：the control group,the model 1 group irradiated by 3 Gy× 8 times and the model 2 group irradiated by 6 Gy × 4 times. Complete blood count, thymus weight index, spleen weight index, activity of SOD, the contents of MDA and lipofuscin were determined.The expression of β-galactosidase was evaluated by staining at the end of 50 days experiment. RESULTS： Compared with control group, the complete blood count, thymus weight index and spleen weight index all decreased（P 〈 0.05）, the activity of SOD also decreased（P 〈 0.05）, but the contents of MDA and lipofuscin were increased（P 〈 0.05） .The β-galactosidase activity of hepatic cells was enhanced in the aging mice model. CONCLUSION： The acute aging model by γ-ray irradiation was successfully established, and it was a feasible method.
出处
《癌变．畸变．突变》
CAS
CSCD
2007年第6期491-493,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
衰老
自由基
模型
aging
free radical
model
