我国急性散发性戊型肝炎病毒部分核苷酸序列分析

Partial nucleotide sequencing of hepatitis E virus isolated from sera of patients with acute sporadic hepatitis E in China

用逆转录套式聚合酶链反应（ＲＴ－ｎＰＣＲ）自深圳、长春、杭州等地４１份急性散发性戊型肝炎病人血清中获得２８株ＨＥＶｃＤＮＡ，对其中３株ＨＥＶｃＤＮＡ的ＯＲＦ２基因片段，用荧光法直接测定其核苷酸序列，并与戊型肝炎病毒墨西哥株（Ｍ）、缅甸株（Ｂ）和新疆流行株（ＣＨ１·１）进行了比较，结果该３株散发性ＨＥＶ与Ｍ株的核苷酸序列同源性分别为８０．２％、７９．９％和７９．４％；与Ｂ流行株的同源性为９５．５％、９３．９％和９５．１％；与散发株的同源性为９３．４％、９２．３％和９３．８％；与ＣＨ１·１的同源性为９７．０％、９６．５％和９５．９；表明该３株散发性ＨＥＶ与ＨＥＶ（Ｂ）和ＣＨ１·１的核酸序列同源性较高，可能属同一亚型。

hepatitis E virus (HEV) cDNA isolates were obtained from 41 sera of patients with acute sporadic hepatitis E (HE) in Shenzhen, Changchun and Hangzhou by a reverse transcription nested polymerase chain reaction (RT-nPCR) and 3 of the 28 RT-nPCR products of HEV cDNA ORF2 region were sequenced. The nucleotide homology of these three HEV isolates was 80.2%, 79.9% and 79.4%, respectively, as compared with the Maxico strain of HEV (M); 93.4%, 92.3% and 93.8% with the Burma epidemic strain of HEV(B) (Ep); 95.5%, 93.9% and 95.1% with the Burma sporadic strain of HEV(B) (Sp); 97.0%, 96.5% and 95.9% with the Xinjiang epidemic strain of HEV CH 1·1. It is demonstrated that these three isolates of HEV obtained from sera of acute sporadiic hepatitis E cases have a higher degree of homology with the Xinjiang strain of HEV CH 1·1 and the Burma strain of HEV(B). The data indicate that these three HEV isolates may belong to HEV(B) subtype, as the HEV CH 1.1 does.