用聚合酶链反应检测食管癌组织中人乳头瘤病毒DNA

Detection of human papillomavirus DNA sequence in esophageal carcinoma using polymerase chain reaction

应用聚合酶链反应（ＰＣＲ）技术对汕头市区６８例食管癌的石蜡包埋标本进行人乳头瘤病毒（ＨＰＶ）ＤＮＡ序列检测，结果显示，ＨＰＶＤＮＡ总阳性率为６６．１８％（４５／６８），检出型别主要为ＨＰＶ６、１１、１６，检出率分别为２７．９４％、３６．７６％和２７．９４％，经统计学处理三型间无显著性差异；ＨＰＶ－１８及未定型别各占８．８２％。值得注意的是ＨＰＶ感染中多重感染占阳性病例的５３．３３％（２４／４５）。初步结果表明，汕头市食管癌高发区有较高的ＨＰＶ感染率，此与食管癌的发生，可能有密切关系。

Human Papillomavirus (HPV) DNA sequence in 68 paraffin-embedded specimens of esophageal carcinoma collected from high-risk Shantou City was detected and typed by polymerase chain reaction (PCR) technique. The results showed a 66 18% (45/68) positive rate of HPV DNA. Among these esophageal carcinoma specimens, 44 out of the 45 positive specimens were squamous cell carcinoma and one was adenosquamous carcinoma. The HPV DNA types detected were mostly HPV-6, -11, and -16. HPV-6 DNA was found in 19(27.94%) of the 68 specimens, HPV-11 DNA in 25(36.76%) and HPV-16 in 19(27.94%) specimens. Among these three types, the difference gave no statistical significance (P>0.05). But HPV-18 DNA was detected in only 6 (8.82%) specimens and the HPV in other 6 specimens was unidentified. It should be noted that as many as 24 (53.33%) of the 45 positive cases had multi-infection (coinfection of 2 or 3 types). Our results suggested that a higher detectable rate of HPV DNA was found in Shantou City as a high-risk region and that HPVs might be closely associated with the pathogenesis of the esophageal carcinoma.