柯萨奇B3病毒诱导小鼠单核-巨噬细胞产生肿瘤坏死因子和白细胞介素6

Coxsackievirus B3-induction of tumor necrosis factor and IL-6 of murine monocyte-macrophages

观察柯萨奇Ｂ３（ＣｏｘＢ３）病毒对小鼠单核－巨噬细胞（Ｍｏ／Ｍφ）的感染，及诱导白细胞介素６（ＩＬ－６）和肿瘤坏死因子（ＴＮＦ）的产生。发现ＣｏｘＢ３能够感染小鼠腹腔巨噬细胞，并释放感染性病毒颗粒；一定感染量病毒的感染，对Ｍｏ／Ｍφ的存活率无明显影响。在体内和体外实验中，ＣｏｘＢ３的感染均可明显诱导Ｍｏ／Ｍφ释放单核因子，在受染的Ｍｏ／Ｍφ培养上清液中及ＣｏｘＢ３感染急性期小鼠血浆和心肌匀浆液中均可测到ＩＬ－６和ＴＮＦ浓度明显升高。

This study is to observe the infection of coxsackievirus B3 (Cox B3) to murine monocyte-macrophages (Mo/Mφ), and its induction of tumor necrosis factor (TNF) and IL-6. Cox B3 was capable of infecting murine macrophages as revealed by immunofluorescence and releasing infectious virus particles. Virus infection with ≤100 TCID50 did not reduce macrophages viability. Cox B3 stimulated the release of monokines from Mo/Mφ in vitro and in vivo, markedly increasing the concentration of TNF and IL-6. These were found in the plasma and homogenized heart tissue during the acute phase of murine myocarditis induced by Cox B3, and in the culture supernatant of Mo/Mφ infected by Cox B3.