二神丸与四神丸对脾虚泄泻大鼠分泌型IgA白介素-2含量影响的配伍比较研究

Study of Different Ershen Pill and Sishen Pill on Wistar Rat of Diarrhea of the Contents of SIgA and IL-2

目的:探讨二神丸、二神丸去姜枣和四神丸对脾虚泄泻作用机制的配伍组方差异性。方法:建立大黄致脾虚泄泻模型,设立空白组、模型组、参苓白术散、二神丸去姜枣、二神丸、四神丸和四神汤共7组。放免法检测大鼠十二指肠黏膜分泌型IgA(SIgA)、白介素-2(IL-2)的含量。结果:各给药组的SIgA、IL-2含量均较模型组升高。结论:二神丸、二神丸去姜枣、四神丸(汤)均能通过升高SIgA分泌量和IL-2含量,共同恢复细胞免疫和体液免疫功能而起作用,在升高脾虚泄泻大鼠白介素-2含量方面,四神汤最优,其次为二神丸组,二神丸去姜枣最差;在升高SIgA含量方面,二神丸最优,二神丸去姜枣最差。其作用效果之差异性可能与以下因素有关:(1)在升高IL-2含量方面,二神丸与五味子散配伍后体现了最佳的协同作用;肉豆蔻、补骨脂与生姜、大枣配伍后亦体现了较好的协同作用;但单独应用补骨脂、肉豆蔻协同作用并不显著。(2)在升高SIgA含量方面,肉豆蔻、补骨脂与生姜、大枣具有协同作用。(3)在提高机体免疫力,调节全身免疫机能方面,生姜和大枣有重要的配伍作用。

Objective： To explore the different functions of ershen pill （ESP） ,＂ subtract ginger and jujube from sishen pill＂ （SGJSSP） and sishen pill （SSP） in treating diarrhea. Methods： Wistar rat model of diarrhea was induced with rhubarb decoction. The contents of SIgA and IL - 2 were detected by radioimmunorassay. Results ： Compared with those of model group,the contents of SIgA and IL -2 in wistar rats are significandy increased. Conclusion： The effect of SGJESP, ESP and SSP on wistar diarrhea of rats can increase the levels of SIgA and IL -2 to improve the humoral immunity and cellular immune function through which the three prescriptions could treat them. , the difference of them may have to do with the factors following： （1）ershen pill and schisandra chinensis baill powder perform an excellent coordination in increasing the of IL-2content. ;Malaytea Scurfpea Fruit,Myristica fragrans Houtt and Ginger,jujube also perform an coordination in increasing the of IL- 2 content; Malaytea Scurfpea Fruit, bfyristica fragrans Houtt don t perform an excellent coordination （2） ;Malaytea Scurfpea Fnalt, Myristica fragrans Houtt and Ginger,jujube also perform an coordination in increasing the of SIgA content; （3） Ginger and jujube are the important factors in increasing the contents of SIgA and IL - 2.