胡枝子醇提工艺的研究

Study on the Ethanol Extraction Process of Lespdeza bicolor Turcz

以总黄酮量为指标,对胡枝子醇提浓度进行选择试验;考查了水、40%乙醇、55%乙醇、70%乙醇、85%乙醇提取液中总黄酮的提取量,采用紫外分光光度法测定总黄酮含量,并绘制了曲线图;结果表明胡枝子醇提以55%～60%乙醇提取为佳。以总黄酮量、浸膏得率为指标,对胡枝子醇提工艺参数进行选择试验;采用正交试验法考查了加醇量、提取次数、提取时间对提取液中总黄酮提取量、浸膏得率的影响,采用紫外分光光度法测定总黄酮含量,并进行了验证试验;结果表明胡枝子醇提以55%～60%乙醇回流提取2次,第1次加7倍量,回流2.0h,第2次加5倍量水,回流1.5h提取为佳。

Take the total flavoues as referance, select the rate of ethanol of the extraction of Herba Lespdeza. , to dewell on the extracting quanitity of total flavoues in the water, 40% ethanol, 55% ethanol, 70% ethanol, 85% ethanol extracting solution, applying UV method to determine the total flavoues content, moreover, describe the curve tab. The result showed that the optimum extracting rate of ethanol of Herba Lespdeza. is 55% - 60%. Take the total flavoues as referance, select the rate of ethanol of the extraction of Lespdeza bicolor Turcz, applying Orthogonal Design method to dewell on the extracting quanitity of total flavoues in the water, 40% ethanol, 55% ethanol, 70% ethanol, $5% ethanol extracting solution, applying UV method to determine the total flavoues content, moreover, describe the curve tab. The result showed that the optimum extracting rate of ethanol of Lespdezabicolor Turcz is 55% - 60%.