模拟失重对MG-63细胞形态、增殖及周期的影响

Effects of Simulated Weightlessness on Cellular Morphology,Cell Multiplication and Cell Cycle of MG-63 Cells.

目的探讨模拟失重对人骨肉瘤成骨样细胞(MG-63)细胞面积、增殖、周期的影响。方法采用回转器模拟失重,用图像处理分析软件(Image J)测量分析模拟失重后细胞面积的改变,用细胞计数法测定细胞的生长曲线,用流式细胞仪测定细胞周期。结果回转器模拟失重后,回转组MG-63细胞24h、36h和48h时间段的细胞面积较对照组缩小(P<0.05),回转组24h、36h、48h3个时间段与12h时间段比较细胞面积缩小(P<0.05),而对照组各时间段之间无明显差异。细胞生长曲线显示模拟失重后细胞增殖明显受抑制。12h、36h、48h回转组MG-63细胞的细胞周期与对照组相比无明显差异,24h回转后G0+G1期细胞显著减少(P<0.05),S期细胞则显著增多(P<0.05)。结论回转器模拟失重使MG-63细胞在24h、36h和48h细胞面积缩小,12h、24h、36h和48h增殖生长受到抑制,24h时出现细胞周期阻滞。

Objective To investigate the effects of simulated weightlessness on cell area, cell multiplication and cell cycle of MG-63 cells. Methods The rotating clinostat was used to simulated weightlessness. The change of cell area was measured with image processing and analysis soft （Image J）. Cell growth curve was measured with the cytometry. The change of cell cycle was examined with a flow cytometer. Results Compared with control group, the cell area of cell line MG-63 in 24 h,36 h and 48 h duratons of rotating group decreased significantly （P 〈0.05）. Compared with 12 h duration, the cell area in 24 h, 36 h and 48 h durations of rotating group decreased significantly （P 〈 0.05）. But in control group, cell area of all 4 experimental durations showed no significant difference among each other. Compared with control group, cell growth curve revealed that cell multiplication was inhibited in rotating group. Compared with control group, the cell cycles of cell line MG-63 in 12 h, 36 h, 48 h sections of rotating group did not change significantly, but the cell in 24 h duration of rotating group was decreased significantly （P 〈0.05） in Go ＋ G1 phase and increased significantly （ P 〈0.05 ） in S phase, Conclusion Simulated weightlessness in rotating clinostat may lead cell area of cell line MG-63 in 24 h, 36 h and 48 h durations to be decreased. The cell multiplication is inhibited in 12 h, 24 h, 36 h and 48 h durations, The conversion of cell from S phase to G2 phase is inhibited in 24 h duration.