DDPH对实验性高血压大鼠血管平滑肌细胞增殖及对PDGF-B,bFGF,c-sis,c-myc的影响

Effects of 1 (2, 6 dimethylphenoxy) 2 (3, 4 dimethoxyphenylethylamino) propane hydrochloride on proliferation of vascular smooth muscle cells and PDGF B, bFGF, c sis, c myc in experimental hypertensive rats

目的：观察１（２，６二甲基苯氧基）２（３，４二甲氧基苯乙胺基）丙烷盐酸盐（ＤＤＰＨ）对实验性高血压大鼠血管平滑肌细胞（ＶＳＭＣ）增殖的作用及对生长因子ＰＤＧＦＢ，ｂＦＧＦ及其相关癌基因ｃｓｉｓ，ｃｍｙｃ表达的影响。方法：采用氚胸腺嘧啶核苷（３ＨＴｄＲ）掺入法，电镜，免疫组化，原位杂交法进行实验观察。结果：ＤＤＰＨ在降低血压同时，能减少ＶＳＭＣ的线粒体，粗面内质网和３ＨＴｄＲ掺入量，并能逆转ＶＳＭＣ增殖时ＰＤＧＦＢ，ｂＦＧＦ抗原及ｃｓｉｓ，ｃｍｙｃｍＲＮＡ的表达增强。结论：ＤＤＰＨ能抑制实验性高血压大鼠的ＶＳＭＣ增殖，与生长因子及癌基因调控的分子生物学机制有关。

OBJECTIVE: To determine the effects of 1 (2, 6 dimethylphenoxy) 2 (3, 4 dimethoxyphenylethy lamino) propane hydrochloride (DDPH) on proliferation of vascular smooth muscle cells (VSMC) and the expression of growth factor PDGF B, bFGF and oncogene c sis, c myc in experimental hypertensive rats. METHODS: 3 H thymidine ( 3 H TdR) incorporation, electronic microscopy, immunohistochemistry and in situ hybridization assaies were used. RESULTS: After administration of DDPH, morphological change of VSMC was antagonized, and blood pressure and 3 H TdR data were significantly reduced. Furthermore, DDPH reversed the enhanced expression of antigen PDGF B, bFGF and reduced expression of oncogene c sis, c myc in experimental hypertensive rats. CONCLUSION: These results suggested that DDPH might inhibit proliferation of VSMC in experimental hypertensive rats, related to the mechanism of molecular biology of controlling growth factors and oncogene.