120名中国人磺胺二甲嘧啶N-乙酰化表型分析

N acetyltransferase phenotype in 120 Chinese using sulfamethazine as the probe drug

目的：对１２０名健康志愿者进行了Ｎ乙酰化表型分析。方法：以磺胺二甲嘧啶（ＳＭ２）为探针药物，用ＨＰＬＣ测得受试者３ｈ尿样，６ｈ后尿样及血样中ＳＭ２及乙酰化磺胺二甲嘧啶（ＡｃＳＭ２），以ＡｃＳＭ２摩尔百分数（ＡｃＳＭ２％）为分型指标。结果：作频数图后，受试者均呈明显两态分布，３种样品中ＡｃＳＭ２％值小于７２％，８５％，５０％者被分为慢代谢者，１２０名受试者中慢代谢者分别为２２，２０，２１人，对３种样品的相关分析表明方法之间相关性达０．９以上。结论：经综合分析，２０名受试者可明确划分出慢代谢者，占１６．６％。此研究结果将为进一步探讨应用咖啡因作为探针药物以及进一步的基因分析打下了一定基础，同时也有助于进一步明确中国人Ｎ乙酰化分型的情况。

OBJECTIVE: To determine the phenotype of 120 Chinese. METHODS: The sulfamethazine (SM 2) was used as probe drug. The urine sample of 3, 6 hours and blood sample of 6 hours were measured with high performance liquid chromatography. The molar percent of its metabolite N acetylsulfamethazine (Ac SM 2) was used as phenotyping index. RESULTS: The frequency distribution of molar percent of three urine samples of 3 hours, 6 hores, and blood of 6 hours indicated a bimodal distribution. On the basis of an assumed antimode of 72%, 85%, 50%, 22,20, and 21 of the 120 subjects were designated as slow acetylators respectively. The correlation of the three samples were all above 0.9. CONCLUSION: 20 subjects (16.6%) can be classified as slow acetylators definitely. The N acetyltransferase phenotype results will be helpful for comparing with genotgping results.