摘要
介绍了用尿素法提取蜘蛛基因组DNA。通过与其他DNA提取方法相比较,证明尿素法具有可在室温条件下进行、DNA得率高、完整性好、简单快速等优点。以提取的DNA为模板进行PCR扩增,获得预期大小的、高重复、高GC含量的编码蜘蛛牵引丝蛋白基因的DNA片段。
The present paper deals with the urea lyses method for isolating spiders＇ genomic DNAs. Compared to other two known methods, this method produced spider genomic DNAs with higher yields and higher integrity. It is also simpler and less time-consuming, involving only room temperature manipulations. High quality genomic DNAs were isolated from spider by this method. Certain spider dragline gene fragments, which contain highly repetitive sequences of high GC contents,were successfully PCR-amplified.
出处
《动物学杂志》
CAS
CSCD
北大核心
2007年第6期64-68,共5页
Chinese Journal of Zoology
基金
国家高技术研究发展计划(863计划)资助项目(No2006AA03Z0451)
