一种恶性疟原虫保护性抗原重组复合基因表达产物的细胞免疫特性研究(I)

Cellular immune response to a hybrid Plasmodium falciparum recombinant antigen (I)

目的：检测一种恶性疟原虫保护性抗原重组复合基因表达产物（ＨＲＡ）诱导小鼠细胞免疫的反应．方法：免疫小鼠的脾淋巴细胞，体外分别经ＣｏｎＡ、ＨＲＡ和可溶性裂殖子抗原（ＳＭＡ）的刺激，３Ｈ－ＴｄＲ参入法检测淋巴细胞的增殖水平．结果：含佐剂的ＨＲＡ免疫ＢＡＬＢ／ｃ和Ｃ５７ＢＬ／６小鼠后，其脾细胞经ＨＲＡ刺激后，增殖明显，可溶性裂殖子抗原免疫的ＢＡＬＢ／ｃ小鼠脾细胞经ＨＲＡ刺激发生明显的增殖．结论：ＨＲＡ上的Ｔ细胞位点发挥了效应．具有一定的突破ＭＨＣ限制的能力．

Objective: The cellular immune responses to a Plasmodium falciparum hybrid recombinant antigen (HRA) were investigated. HRA codes for several T/B cell epitopes from MSA1, MSA2, RESA and Universal T cell epitopes of IL 1, tetanus toxiod. Methods: Splenocyte proliferation of immunized mice was examined by 3H TdR incorporation. Results: HRA could prominently induce splenocyte proliferation of BALB/c and C57BL/6 mice after immunized with HRA emulsified with Freund adjuvant.HRA could also induce prominently proliferation BALB/c mice immunized with soluble merozoite antigen (SMA). Conclusion: T cell epitopes of HRA are effective. HRA may overcome MHC restriction.