增殖细胞核抗原表达与AgNORS计数在胆囊癌中的意义

PCNA expression and AgNORS count in primary gallbladder carcinoma and its significance

目的：研究增殖细胞核抗原表达与ＡｇＮＯＲＳ计数在胆囊癌中的意义．方法：应用抗增殖细胞核抗原（抗ＰＣＮＡ）单克隆抗体，采用ＡＢＣ免疫组化方法和ＡｇＮＯＲＳ染色计数，对４０例原发性胆囊癌进行了研究．结果：ＰＣＮＡ表达阳性率与ＡｇＮＯＲＳ计数间存在着明显的相关性（Ｐ＜０．０５）．二者均随分化程度的增高而减小；与淋巴结转移有关；术后存活３年或３年以上者明显小于３年以内者（Ｐ＜０．０１）．

Objective: To study PCNA expression and AgNORS count in primary gallbladder carcinoma and its significance. Method: The expression of PCNA and AgNORS count in 40 cases of primary gallbladder carcinoma were studied by ABC immunostaining of monoclonal antibody (PC 10). Results: The positive rate of PCNA expression was evidently correlated with result of AgNORS count. Both were related to the histological type and lymph node metastasis, decreased with the increasing degree of histological differentiation. Survival analysis showed that the positive rate of PCNA expression and the results of AgNORS count in operated patients with survival time≥3 years were significantly lower than those with survival time <3 years (P< 0.01 ). Conclusion: Immuno staining of PCNA and AgNORS count are helpful to judging the malignant degree and prognosis of primary gallbladder cancer.