液-质联用在西罗莫司和依维莫司血药浓度监测中的应用进展

Development of LC-MS application in measurement of sirolumus and everolimus

西罗莫司和依维莫司是一种新型的免疫抑制剂,相对于环孢霉素(CsA),西罗莫司和依维莫司服用方便、吸收好、起效快,但也存在着用药剂量小、个体吸收差异大、治疗窗窄.因此,测定个体血药浓度以调整用药的剂量和次数、制定个体化治疗方案在临床上有着至关重要的意义.本文就近几年液-质联用在西罗莫司和依维莫司血药浓度监测中的应用研究作以简要综述,以期为临床合理用药提供参考.

Sirolimus and Everolimus is used in posttransplantation patient care and autoimmune disease therapy. Because of its narrow therapeutic ranges and high variations of the interindividual phannaeokineties, therapeutic drug monitoring （TDM） of Sirolimus and Everolimus is critical. The most common methods used in the clinical laboratories for measurement of are liquid chromatography coupled with ultraviolet detection, which are subject to interference by the metabolites formed in vivo. High - performance Liquid chromatography coupled with mass spectrometry （ LC - MS） is a powerful analytical tool that provides high specificity and sensitivity in TDM of Sirolimus and Everolimus. In this article, the recent development of LC - MS application in measurement of Sirolimus and Everolimus is reviewed in order to afford some useful information of clinically choosing medicine.