摘要
目的:探讨TGF-β1和IGF-Ⅰ在兔骨关节炎(OA)模型软骨细胞中的表达及意义。方法:新西兰兔40只,随机分成实验组和对照组各20只。实验组选用Hulth法造模,对照组行单侧膝关节切开,两组分别于术后4、8周各处死10只。取术肢股骨髁标本行HE染色镜下观察软骨细胞排列情况;免疫组化染色检测TGF-β1和IGF-Ⅰ的表达情况,并应用镜下观察结合病理图像分析系统计算各组软骨细胞TGF-β1和IGF-Ⅰ表达的细胞分数。结果:(1)HE染色:实验组软骨细胞排列分层混乱,细胞稀疏,有软骨缺损;部分软骨细胞集聚、肿胀,且可见大量空陷窝。(2)免疫组化染色:TGF-β1和IGF-Ⅰ在两组中均有表达,而实验组细胞分数均明显高于对照组(P<0.01);实验组的软骨缺损部位细胞分数均明显高于软骨完整部位细胞分数(P<0.01),造模后8周细胞分数较4周明显增高(P<0.01)。结论:OA时TGF-β1和IGF-Ⅰ的表达均增多,且随软骨缺损程度的加重表达增高明显,软骨无修复。其机制需进一步研究。
Objective： To investigate the expression and the significance of TGF-β1 and IGF-I in the chondrocyte of OA model in rabbit. Methods： There were forty New Zealand rabbits, they were randomly divided into experiment group and control group. There were twenty in each group. The model of OA was established with Hulth＇s method in experiment group, the rabbits of control group received unilateral knee arthrotomy. There were ten rabbits were killed in each group at four and eight weeks after the operation. H-E stains were adopted. The expression of TGF-β1 and IGF- I were detected through Immunohistochemistry. The cell fraction of TGF-β1 and IGF- I was recorded by image analytical system. Data were analyzed through PEMS3.1 statistical software. Results： （1）H-E stains： The chondrocytes in control group were regular, welllayer and homogeneous. While in experiment group, chondrocytes were swollen,irregular and coloboma at 4 weeks： chondrocytes were sparse, some assembled and swollen,a large amount of vacuolar lacuna were seen, and more coloboma in cartilagines at 8 weeks; （2） Immunohistochemistry： There was the expression of TGF-β1 in two groups,and The cell fraction in experiment group was more than in control group of the same period（P〈0.01）. In experiment group,the cell fraction of defective locality of cartilagines was higher than that in intact locality of cartilagines at 4 weeks and 8 weeks（P〈0.01）. The cell fraction in the experiment group （defective locality,intact locality of cartilagines）at 8 weeks was much higher than that of 4 weeks （P〈0.01）. There was also the expression of IGF- I in two groups. And the cell fraction in experiment group was more than that in control group of the same period （P〈0. 01）. The cell fraction of defective locality of cartilagines in experiment group at 4 weeks and 8 weeks was much more than that of intact locality （P 〈0.01）; The cell fraction at 8 weeks in experiment group （ the defective locality, intact locality of cartilagines） was much more than that of 4 weeks （P〈0.01）. Conclusion： The expression of TGF-β1 and IGF- I of OA increased. The expression was increasing as the defection of the cartilagines got worse. The cartilage showed no repair. Further study is needed to investigate the mechanism.
出处
《中国冶金工业医学杂志》
2007年第6期637-640,F0003,共5页
Chinese Medical Journal of Metallurgical industry
