VEGF反义核酸抑制肿瘤血管形成的研究

Inhibition of angiogenesis with antisense ODN of VEGF

目的探讨ＶＥＧＦ反义核酸作为抗新生血管形成药物和抗肿瘤药物的可能性。方法在小鼠角膜肿瘤模型上，测定两种ＶＥＧＦ反义核酸序列的５种不同修饰之反义核酸，对肿瘤血管形成和肿瘤生长的抑制作用。结果线性片段疗效较差，部分硫代和发夹结构两种修饰的反义核酸疗效满意。两种序列反义核酸混合使用最好。结论ＶＥＧＦ反义核酸有望成为抗肿瘤和抗新生血管形成的药物。

Objective To investigate whether antisense oligodeoxynucletides(ODNs) of VEGF can become a kind of drugs for antitumor cancer treatment. Methods With a tumor model on the cornea of rat the inhibition of angiogenesis and tumor growth by 5 ODNs with different modifications and sequences was determined. Results ODNs with partial phosphorothioate modification, or with hairpin structure could effectively inhibit tumor induced angiogenesis, but the linear ODN could not. The use of 2 ODNs at different sites gave the best results. Conclusion VEGF antisense ODNs can hopefully become new drug with antitumor and antiangiogenesis activities.