氯代甲烷对小麦叶绿素和土壤呼吸率的影响

Effects of Chloromethane on Wheat Chlorophyll and Soil Respiration

采用室内模拟实验方法,研究了二氯甲烷(DCM)、三氯甲烷(CF)、四氯化碳(CT)等氯代甲烷对小麦幼苗叶绿素含量和土壤呼吸率的影响。结果表明:不同浓度的氯代甲烷对小麦叶绿素的合成有不同程度的抑制作用,且浓度越高,抑制强度越大。DCM、CF、CT对叶绿素的半抑制浓度分别是399.3 m g/kg,42.82 m g/kg,9.14 m g/kg,说明对叶绿素毒性作用CT>CF>DCM,即甲烷中氢原子被取代的越多,其毒性越大。3种氯代甲烷对土壤呼吸率都显示出了抑制作用,抑制率随浓度的增加而增加,但氯代甲烷对土壤呼吸率的抑制是暂时的,随着时间延续,土壤呼吸率会有所恢复。

The simulated experiments were conducted to study effects of chloromethane included DCM,CF and CT on wheat seedling chlorophyll and soil respiration.The results showed that,the wheat chlorophyll were inhibited,and the inhibition was varied when the concentration of chloromethane was different.The more concentration was,the lower content of wheat chlorophyll was.The median inhibitory concentrations of DCM,CF and CT were 399.3 mg/kg,42.82 mg/kg,9.14 mg/kg,which indicated the toxicity of CT 〉 CF 〉 DCM.That was to say,the more H atoms were replaced,the more toxin would be.All the three chloromethane inhibited the soil respiration,and the inhibitions were stronger when the chloromethane concentrations increased.The inhibitory effects to soil respiration were temporary and would renew as the time last.