Bcl-2反义寡核苷酸对环已亚胺诱导U251细胞凋亡的影响

Effect of Bcl-2 antisense oligodeoxynucleotides apoptosis of U251 glioma cells induced by cycloheximide

目的探讨Bcl-2反义寡核苷酸对环已亚胺(CHX)诱导胶质瘤细胞U251凋亡的影响。方法在光镜下观察Bcl-2反义寡核苷酸、CHX及二者联合应用对U251凋亡的形态学改变;应用MTT法检测各组U251细胞的抑制率;通过DNA电泳检测CHX诱导U251细胞凋亡的情况。结果Bcl-2反义寡核苷酸可明显促进CHX诱导的U251凋亡,对照组、SODN组与CHX组、AODN组、SODN+CHX组及AODN+CHX组的细胞抑制率相比P均<0.01,AODN组、SODN+CHX组和AODN+CHX组的细胞抑制率相比P均<0.01;Bcl-2反义寡核苷酸可促进细胞凋亡,呈现出特征性的DNA梯状带。结论Bcl-2反义寡核苷酸和CHX均可诱导胶质瘤细胞U251发生凋亡,且Bcl-2反义寡核苷酸可明显促进CHX诱导的胶质瘤细胞U251凋亡,提高胶质瘤细胞对CHX的敏感性。

[ Objective] To investigate the effect of Bcl-2 antisense oligodeoxynueleotides（AODN） on the apoptosis of human glioma cell lines U251 induced by cycloheximide（CHX）. [ Methods] Morphological changes of U251 cells treated with Bcl-2 antisense oligodeoxynueleotides and CHX alone or combined were detected by light microscope. MTT assay were used to investigate the inhibition ratio of different groups. Meantime, apoptosis was identified by DNA electrophoresis. [ Results] Bel-2 antisense ohgodeoxynueleotides could obviously enhances the apoptosis of U251 cells indeeed by CHX. The apoptosis rates of CHX group, AODN, SODN ＋ CHX and AODN ＋ CHX were higher than those of control group and SODN group （P 〈 0.01 ）. The differences among AODN group, SODN ＋ CHX group and AODN ＋ CHX group had statistical significance（P 〈 0.01 ）. Bel-2 ASON make U251 cells more sensitive to CHX. DNA ladder was viewed on agarose gel. [ Conclusion ] Both Bcl-2 AODN and CHX can enhance the apoptosis of U251 cells. Bel-2 AODN can obviously enhance the apoptosis of U251 cell induced by CHX.