摘要
采用根癌农杆菌介导法将bar基因导入粳稻恢复系H84中,获得12棵转基因阳性苗。T1个体的叶片除草剂抗性鉴定结果表明:8个群体的分离比符合3∶1分离;对T3株系进行芽苗的除草剂抗性鉴定,从中获得了10个抗性表现为纯合的株系。用这些株系与1513A不育系杂交,并分别鉴定和测定了F1杂种的除草剂抗性、纯度、育性和主要农艺性状,结果表明:F1杂种对Basta除草剂均表现为抗性,F1的育性和主要农艺性状与对照相比没有明显差异,显然bar基因的插入对该恢复系的恢复力和与不育系的配合力没有影响,同时显示bar基因的应用,对控制水稻杂交种的纯度和田间杂草以及提高产量有着积极意义。
The twelve positive transgenic plants were obtained through transferring bar gene to rice （Oryza sativa L. ssp. keng Ting） restoring line （H84） with Agrobacterium. The test for T1 individuals＇resistance to herbicides showed that the segregation ratio of 8 populations was 3 ： 1, and the 10 isogenic restoring lines in resistance were selected through the test for T3 plantlets＇ resistance to herbicides. These lines were crossed with the sterile line 1513A. The tests for their F1＇s resistance to herbicides, purity, fertility and main agronomic characters showed that F1 hybrids were resistant to Basta herbicide,F1＇s fertility and main agronomic characters were not significantly different from those of check line, and the bar gene insertion had no effect on the restoring line＇s restoring ability and combining ability. The application of bar gene had a positive significance in controlling rice hybrid＇s purity and field weeds and improving rice yield.
出处
《上海农业学报》
CSCD
2007年第4期21-25,共5页
Acta Agriculturae Shanghai
