摘要
目的探讨脑干损伤后立即早基因c-fos、c-jun的表达在脑干损伤法医学鉴定中的意义。方法采用重物自由落体式建立大鼠脑干损伤快速死亡模型,70只大鼠分为两组,实验组(40只),对照组(30只)。肉眼和HE染色观察脑干组织的形态改变,并通过原位杂交的方法检测大鼠脑干损伤部位c-fos、c-jun基因的表达情况。结果100倍光镜视野下实验组c-fos和c-jun mRNA阳性细胞数计数分别为53.62±9.84和46.85±7.43;对照组c-fos和c-junmRNA阳性细胞数分别为0.9±0.87和1.3±0.67;经统计分析,实验组c-fos、c-jun mRNA基因表达水平较对照组显著增高。结论脑干损伤后损伤部位c-fos、c-jun mRNA基因表达水平增高对脑干损伤快速死亡的法医学鉴定具有参考价值。
Objective To study the changes of the immediate early genes e-fos and e-jun mRNA expression in the injured brainstem and explore its medieolegal significance. Methods An animal model in which the rats quickly died of minor brainstem injury was established by using a gravitational force of a free falling weight. Seventy rats were divided into two groups group （30 rats）. The changes of the injured brainstems ：experimental group （40 rats） and normal control were observed by HE staining. The expression levels of c-fos and c-jun were determined by Hybridization in suit. Results The numbers of c-fos, and c-jun mRNA positive ceils at low power were 53.62 ± 9.84 and 46.85 ± 7.43 respectively in the experimental group ; 0.9 ± 0.87 and 1.3 ± 0.67, in the control group. The expression levels of c-fos, and c-jun mRNA gene in the experimental group were obviously higher than in the control one by statistic analysis. Conclusion The expression level of c-fos and c-jun mRNA genes of the injured part is helpful to medicole- gal expertise in the case of rapid death after brainstem injury.
出处
《中国法医学杂志》
CSCD
2007年第6期372-375,共4页
Chinese Journal of Forensic Medicine
基金
北京市科技新星计划资助项目(9558102400)
