RP-HPLC测定藏药邦贝抗炎栓中的盐酸小檗碱

Determination of berberine hydrochloride in Tibetan medicine Bangbei Kangyan suppository by RP-HPLC

目的建立测定藏药邦贝抗炎栓中盐酸小檗碱含量的方法。方法采用RP—HPLC法，色谱柱为Kromosil C18（200mm×4．6mm，5μm），流动相为0．1％磷酸-乙腈（65：35），流速1ml·min^-1，检测波长346nm。结果盐酸小檗碱质量浓度0．34～1．68mg·ml^-1与峰面积呈良好的线性关系（r=0．9998），平均回收率为99．7％，RSD=1．25％。结论所建方法简便、准确、重复性好，适用于藏药邦贝抗炎栓制剂中盐酸小檗碱的含量测定。

OBJECTIVE To establish a method for determination of berberine hydrochloride （Bet） in Tibetan medicine Bangbei Kangyan suppository. METHODS RP - HPLC was adopted. 200 mm×4. 6 mm column packed with C18 and mobile phase consisted of 0. 1% phosphoric acid solution and acetonitrile （65 ： 35 ）. The eluents were monitored at 346 nm. The flow rate was 1.0 ml·min^-1 RESULTS A good linearity was obtained in the range of 0. 34 - 1.68 mg·ml^-1 （ r = 0. 9998 ）. The recovery was 99. 7 % with the RSD of 1.25 %. CONCLUSION The method is sensitive, accurate and simple. It can be used for control the quality of Tibetan medicine Bangbei Kangyan suppository.