摘要
目的建立测定藏药邦贝抗炎栓中盐酸小檗碱含量的方法。方法采用RP—HPLC法,色谱柱为Kromosil C18(200mm×4.6mm,5μm),流动相为0.1%磷酸-乙腈(65:35),流速1ml·min^-1,检测波长346nm。结果盐酸小檗碱质量浓度0.34~1.68mg·ml^-1与峰面积呈良好的线性关系(r=0.9998),平均回收率为99.7%,RSD=1.25%。结论所建方法简便、准确、重复性好,适用于藏药邦贝抗炎栓制剂中盐酸小檗碱的含量测定。
OBJECTIVE To establish a method for determination of berberine hydrochloride (Bet) in Tibetan medicine Bangbei Kangyan suppository. METHODS RP - HPLC was adopted. 200 mm×4. 6 mm column packed with C18 and mobile phase consisted of 0. 1% phosphoric acid solution and acetonitrile (65 : 35 ). The eluents were monitored at 346 nm. The flow rate was 1.0 ml·min^-1 RESULTS A good linearity was obtained in the range of 0. 34 - 1.68 mg·ml^-1 ( r = 0. 9998 ). The recovery was 99. 7 % with the RSD of 1.25 %. CONCLUSION The method is sensitive, accurate and simple. It can be used for control the quality of Tibetan medicine Bangbei Kangyan suppository.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第6期689-690,共2页
West China Journal of Pharmaceutical Sciences