摘要
[目的]了解河北省SENV的感染情况和基因进化特征。[方法]以SENV读码框架1区(ORF1)核苷酸序列设计引物建立巢式聚合酶链反应(nested-PCR)方法,对306份来自不同人群血清标本进行SENV-D/H亚型检测,并对部分扩增产物进行序列分析。[结果]306份标本中,SENV DNA(D和H亚型)总阳性率为26.8%。健康人群、乙型肝炎、肝硬化患者、急性甲型肝炎、丙型肝炎和非甲-非戊型肝炎组SENV DNA的阳性率分别为22.9%(11/48)、26.0%、16.7%、25.8%、47.6%、40%。丙型肝炎患者和健康人群之间差异有统计学意义(2χ=4.21,P﹤0.05),其他各组与健康人群之间均差异无统计学意义(P﹥0.05),SENV-D和SENV-H DNA的检出率分别为18.6%和17.6%,两型之间差异无统计学意义(P﹥0.05)。5份来自不同人群的SENV-D亚型测序株部分核苷酸序列与标准株(AX025730)同源性≥91.6%,4份来自不同人群的SENV-H亚型测序株部分核苷酸序列与标准株(AX025838)同源性≥88.6%。[结论]在河北省健康人群和肝炎人群中SENV感染均存在,并出现河北省地方毒株。
[Objective]To investigate the prevalence and gene evolution of SEN virus（SENV）in Hebei province.[Methods]Nested polymerase chain reaction（PCR）that based on ORF1 nucleotide sequence was established to detect SENV-D and H in 306 sera.SENV PCR products were analyzed with DNA sequence analysis.[Results]The total positive rate of SENV-D and H in 306 samples was 26.8%.The positive rates of SENV-D and H in samples of healthy subjects,hepatitis B,cirrhosis,acute hepatitis A,hepatitis C and hepatitis non A-E patients were 22.9%,26.0%,16.7%,25.8%,47.6%,40% respectively.The positive rates of SENV between hepatitis C patients and healthy individuals were significantly different（χ^2=4.21,P﹤0.05）,and those of SENV between other group patients and healthy individuals were not different（P﹥0.05）.The detection rates of SENV-D and H DNA in all samples were 18.6% and 17.6% respectively.The homology of nucleotide sequence of SENV-D from 5 products and the normal strain（AX025730）was above 91.6%,and the homology of nucleotide sequence of SENV-H from 4 products and the normal strain（AX025838）was above 88.6%.[Conclusion]There are infections of SENV in healthy individuals and hepatitis patients.The local strains may exist in Hebei province.
出处
《现代预防医学》
CAS
北大核心
2007年第23期4443-4445,共3页
Modern Preventive Medicine
