摘要
基于对沙棘化学成份的调查,沙棘果中75%的乙醇提取物及其枝条中80%的丙酮氯仿可溶性物质可以抑制脂多糖(LPS)和重组小鼠的干扰素(IFN)—γ活性吞噬细胞即RAW264.7细胞中一氧化氮(NO)的生成。从果实提取物中可以分离出三种已知的黄酮、槲皮素(1)、kaempferol(2)、异鼠李素(3)和两种已知的萜类,果酸(4)和熊果酸(5)。该试验第一次从沙棘中提取出果酸,此外还从沙棘树皮的萃取物中分离出一种新的萜类、2—O—caffeoyl—谷酸(6),已知的萜类,鱼肝油醇酸(7),6—甲氧基—2H—1—苯芘(8)和β-醇(9)。并对RAW264.7细胞中的NO产物的活性抑制作用进行了研究。
In our investigation on the chemical constituents of Hippophae rhamnoides L. , the 75 % ethanol extract of fruits and the chloroform soluble fraction of the 80% acetone extract of branch barks were observed to inhibit nitric oxide （NO） production in a lipopolysaccharide （LPS） and recombinant mouse interferon （IFN） -γ activated murine macrophage-like cell line, RAW 264. 7 cells. Three known flavonols, quercetin （1）, kaempferol （2）, and isorhamnetin （3）, and two known triterpenoids, pomolic acid （4） and ursolic acid （5） were isolated from the fruits extract. Pomolic acid was isolated from H. rharnnoides for the first time. On the other hand, a new triterpenoid, 2-O-caffeoyl- maslinic acid （6）, and known triterpenoids, oleanolic acid （7）, 6-Methoxy-2H-1-benzopyran （8）, and β-sitosterol （9） were isolated from the branch barks extract. Their inhibitory activity on the production of NO in RAW 264.7 cells were examined.
关键词
沙棘
2-O-咖啡醇-谷酸
一氧化氮
巨噬细胞
Hippophae rhamnoides L.
2-O-caffeoyl-maslinic acid
nitric oxide
macrophage
