正常妊娠及妊高征患者外周血中IL-12、IL-18的检测意义

The detection significance of interleukin (IL)-18 and IL-12 in peripheral blood of normal pregnant and pregnancy-induced hypertension patients

目的:检测非孕妇女、正常妊娠及妊娠高血压综合征(妊高征,PIH)患者外周血中IL-12和IL-18水平,探讨Th1/Th2细胞因子在妊高征发病中的作用。方法:孕20周后的子痫前期患者34例(轻度子痫前期15例,重度子痫前期19例),正常妊娠组32例,非妊娠对照组16例。取外周血进行流式细胞仪检测,选取CD28表达量最高者的淋巴细胞作为实验对象。采用ELISA法测定IL-12、IL-18,流式细胞仪测定Th1/Th2细胞比。结果:正常妊娠组、非孕组及妊高征组IL-12比较均存在显著性差异(P<0.001)。轻度子痫前期组、重度子痫前期组与正常妊娠组IL-18比较均不存在显著性差异(P>0.05)。各组IL-18/IL-12浓度比比较,正常妊娠组与妊高征组比较存在显著性差异(P<0.0001)。IL-18/IL-12浓度比与Th1/Th2细胞比的相关性比较,呈明显负相关(P<0.01)。结论:正常妊娠的IL-18/IL-12浓度比明显高于妊高征组与Th1/Th2细胞比呈相反变化。

Objective： To investigate the levels of IL- 18 and IL- 12 in peripheral blood of normal pregnant and in pregnancy -induced hypertension patients, and to explore the role of Thl/Th2 cytokines in the pathogenesis of pre - eclampsia. Methods： 34 cases pre- eclampsia patients pregnant 20 weeks （mild pre- eclampsia 15 cases and severe pre- eclampsia 19 cases） , normal pregnancy group 32 eases, non -pregnant controls 16 eases. The peripheral blood was tested by flow eytometry, selected the lymphoeytes with highest expres- sion of CD28 as experimental subject. IL - 12 and IL - 18 were detected by ELISA Thl/Th2 ratios in PBMC was detected by flow eytonetry. Results： IL - 12 in normal pregnancy group, pregnancy - induced hypertension group and non - pregnant group were signifleant difference （P 〈0. 001 ）. IL- 18 in mild pre- eclampsia cases, severely pre -eclampsia cases and normal pregnancy subjects had no sig- nificant difference （P 〉 0. 05 ）. While the ratios of IL - 18/IL - 12 in normal pregnancy group and pregnancy - induced hypertension group had significant difference （ P 〈 0 . 000 1 ）. Thl/Th2 ratios were negatively correlated with the ratios of IL - 18/IL - 12. Conclusion： The raties of IL - 18/IL - 12 were significantly higher in normal pregnant group than pregnancy - induced hypertension group, and Thl/Th2 raties were negatively correlated with the ratios of IL - 18/IL - 12.