摘要
[目的]探讨蒿甲醚对小鼠结直肠癌血管生成的抑制作用。[方法]BALB/c小鼠腋下接种CT-26结直肠癌细胞(2×106个/ml)40只,雌雄各半;随机分为5组,每组8只:低剂量组(蒿甲醚片,每天33.3mg/kg)、中剂量组(蒿甲醚片,每天50.0mg/kg)、中剂量加铁剂组(每天蒿甲醚片50.0mg+硫酸亚铁1.5mg/kg)、高剂量组(蒿甲醚片,每天66.6mg/kg),对照组给予等容量的生理盐水。采用灌胃法持续给药15天,每天1次,末次给药24小时后处死动物。观察记录肿瘤的生长情况,每3天用电子天平称量小鼠的重量,用游标卡尺测量每只小鼠腋下肿瘤长a(mm)和短径b(mm),用Steel公式计算肿瘤体积V(mm3)=0.5ab2。采用免疫组化方法检测肿瘤组织微血管密度。[结果]高、中、低剂量组和中剂量加铁剂组血管计数分别为13±6,31±4,38±5和11±9,生理盐水对照组为49±9;蒿甲醚各治疗组微血管密度均明显低于生理盐水对照组(P<0.05,P<0.01)。[结论]在一定剂量范围内,蒿甲醚能明显抑制小鼠结直肠癌移植瘤血管生成。
[Purpose ]To explore inhibitory effect of Artmeter on colorectal cancel angiogenesis in mice. [Methods ] Forty BALB/c mice （20 male and 20 female） with subcutaneous plantation of CT-26 eolorectal cells （2×10^6） were randomly divided into 5 groups, 8 mice in each. They were low dose group （33.3mg/kg）, middle dose group （50mg/kg）, middle dose plus ferralia （50mg/kg＋1.5mg/kg）, high dose group （66.6mg/kg） and blank control group with normal saline. Treatment with stomach instillation started from day I to day 15. The mice were sacrificed at 24 hours after last treatment. The mice were weighted in Length-path （a mm） and short-path （b mm） of tumor in each mouse was measured by staff gauge every 3 days. Volume of tumor was ealeulated with Steel＇s luminia： V （mm^3） =0.5ab^2. Micro-vascular dense （MVD） was observed and eonntered under the microscopy hy immunohistory, chemistry. [Results] Micro-vascular count value in groups with high, middle, low and middle dose plus ferralia and control was 13±6,31±4,38±5 anti 11±9. and 49±9 respectively. MVD in treatment groups was significantly lower than that in control group （P〈0.05,P〈0.01 respectively）. [Conclusion] Artmeter at certain range of dosage has a significantly inhibitory effects on eolorectal cancer angiogenesis in BALB/c mice.
出处
《中国肿瘤》
CAS
2007年第12期1012-1014,共3页
China Cancer
