小鼠肝脏再生早期胆酸代谢相关基因SHP和Cyp7A1的动态改变

The Expression of Bile Acid Metabolism Related Genes Cyp7A1 and SHP in the Early Stage of Liver Regeneration in Mice

目的探讨小鼠肝脏70%切除术后再生早期,核受体SHP参与调控Cyp7A1基因表达的机制。方法应用逆转录实时定量PCR检测野生型小鼠和FXR-/-小鼠肝脏70%切除术后1h和6hCyp7A1基因和SHP基因的表达。结果2组肝脏Cyp7A1基因表达在术后1h和6h明显降低;SHP基因表达在术后1h显著上升,术后6h急剧下降。结论小鼠肝脏70%切除术后再生早期,非依赖FXR的通路参与胆酸介导的Cyp7A1基因转录的抑制。SHP可通过非FXR介导的途径参与调节Cyp7A1基因的表达。

Objective To investigate the role of nuclear receptor small heterodimer partner（SHP） in the regulation of Cyp7A1 gene expression during the early stage of mice liver regeneration after 70% hepatectomy. Methods The gene expression of Cyp7A1 and SHP in wild type mice（WT） and farnasol X receptor（FXR）^-/- mice at one hour and six hours of post-hepatectomy were checked with RT-real time PCR. Results In WT and FXR^-/- mice,Cyp7A1 mRNA level were significantly reduced at one hour and six hours after 70% hepatectomy. SHP gene expression was dramatically increased at one hour of post operation, but was statistically decreased at six hours. Conclusion In the early stage of mice liver regeneration, the expression of Cyp7A1 gene were suppressed, while the SHP gene expression was increased. SHP might participate in the inhibition of Cyp7A1 expression via a FXR-independent pathway.