紫杉醇对涎腺腺样囊性癌实验性肺转移的抑制作用

Inhibitive Effects of Paclitaxel on the Lung Metastasis of Salivary Adenoid Cystic Carcinoma in vivo

目的观察紫杉醇对人高转移性涎腺腺样囊性癌细胞(ACC-M)在裸鼠体内肺转移的抑制作用。方法22只裸鼠,尾静脉注射接种ACC-M细胞建立实验性肺转移模型,随机分为紫杉醇组和对照组。紫杉醇组于接种肿瘤细胞后第2天起腹腔注射紫杉醇10mg/kg,3d注射1次,共10次;对照组同期腹腔注射等量溶剂。接种6周后处死实验动物。观察发生肿瘤肺转移的裸鼠数目,称体质量;解剖裸鼠肺脏,称质量;记录肺转移灶数目。结果对照组和紫杉醇组相比,肺转移率100%vs54.54%,肺转移结节数(28.64±10.69)vs(15.17±4.83),转移肺湿质量(0.702±0.044)gvs(0.524±0.046)g,2组比较差别均有统计学意义(P<0.05)。结论紫杉醇对ACC-M细胞肺转移具有明显抑制作用。

Objective To investigate the inhibitive effect of paclitaxel on salivary adenoid cystic carcinoma（ACC-M） with high lung metastases potential in nude mice. Methods The nude mice model of pulmonary metastasis was established by injecting of ACC-M cell suspension 0.2 mL from tail vein. 22 mice were randomized into a treated group and a control group. Paclitaxel（10 mg/kg） was intraperitoneal administered to the mice in treated group every three days for ten times. Different treatments were served as control and all mice were sacrificed after 6 weeks. The lung metastasis occuring rates of two groups were detected. Results Compared the control group with paclitaxel-treated group, the lung metastasis rates was 100% and 54.45%, respectively. The metastatic foci on lung surface were （28.64±10.69） and （15.17±4.83）, respectively. The wet weight of metastatic lung was （0. 702±0. 044）g and （0. 524± 0. 046）g, respectively. The difference between two groups was significant （P〈 0.05）. Conclusion Paclitaxel shows a strong inhibitive effect on lung metastasis with ACC-M in vivo of nude mice.