α-亚麻酸特异性脂肪酶产生菌复合诱变前后性质对比

Contrast on the Characteristics of Specialα-linolenic Lipid-producing Strain before and after Multi-mutagenesis

将前期从野生菌中筛选、分离出的具有产生水解α-亚麻酸特异性脂肪酶的菌种为诱变出发菌,经单一及复合诱变,分离出具有较高特异脂肪酶活性的诱变菌株。本试验通过对诱变前后菌株在生长曲线、革兰氏染色、酶活等方面的测定,了解此菌株诱变前后生理生化特性的变化。

The wild stain which could produce hydrolyze alpha-linolenic lipid was separated and screened. After single and combined mutation, the strain with higher hydrolyzing alpha-linolenic lipase was separated. Physiological and biochemical characteristics of the strain were studied by the growth curve, Gram staining and enzyme activity before and after mutagenesis.