郏县红牛CLPG基因的SNP及对生长发育性状的影响

The SNP of CLPG Gene in Jiaxian Red Cattle and Its Effect on Growth and Development Traits

利用PCR-SSCP检测了113头郏县红牛CLPG基因的SNP,发现了AA、AB、AC 3种基因型,测序结果显示AA型在第150和419碱基处分别表现为C和T,为野生型;AB型在第419处发生碱基T→C突变;AC型在第150处发生碱基C→T突变。多态信息含量为0.226,属低度多态。分析了不同基因型与部分生长发育性状的相关性,结果显示,AA、AC基因型个体在体重、体长指标上显著(P<0.05)或极显著(P<0.01)大于AB型个体;在管围指标上,AA基因型个体优于AB基因型个体,差异显著(P<0.05);在坐骨端宽指标上,AA与AB基因型个体之间差异显著(P<0.05),AB与AC基因型个体间差异极显著(P<0.01)。

The SNPs of CLPG gene in 113 Jiaxian Red cattle were detected by PCR-SSCP, and three genotypes （AA, AB and AC） were identified. The sequencing results showed that genotype AA was the wild type with C and T at the position 150 and 419, respectively, genotype AB and AC have mutations T419C, C150T, respectively. The polymorphism information contents （PIC） was 0. 226, which indicated that the polymorphism of this population was relatively low. The associations of different genotypes with some of the growth and development traits were analyzed. The results showed that the individuals with genotype AA and AC had a larger body weight, body length than the individuals with genotype AB （P〈0.05 or P〈0. 01, respectively）, the individuals with genotype AA were better than those with AB in the circumference of cannon bone （P〈0.05）, the individuals with the genotype AB was significantly different with those of AA （P 〈0.05） and AC （P〈0.01） in the hucklebone width, respectively.