高邮鸭PRL基因内含子1的SNP检测及其与产蛋性状的相关分析

SNP Detection of Intron 1 of PRL Gene and Its Associations with Egg-laying Traits in Gaoyou Duck

利用PCR-RFLP技术对高邮鸭催乳素(Prolactin,PRL)基因内含子1进行了SNP检测和测序分析,并对该基因与高邮鸭产蛋性状的相关性进行了研究。结果表明:PRL基因内含子1存在两个DraI酶切位点,其中1个位点具有DraI多态性。该位点由于在1 326位点发生了T→C的碱基突变,产生了AA、AB和BB 3种基因型。基因型BB和等位基因B的频率最高;BB基因型个体30周龄蛋重极显著高于AB基因型个体(P<0.01);AB基因型个体的双黄率显著高于BB基因型个体(P<0.05);3种基因型间产蛋数、最长连产天数和开产体重无显著差异。

In present study, SNP of prolactin（PRL） gene intron 1 of Gaoyou duck were detected by PCR-RFLP and DNA sequencing. The correlation analysis between the polymorphism of PRL gene intron 1 and egg-laying traits was carried out. The results indicated that there were two Dra I digestion sites in intron 1 of PRL gene, but only one was polymorphic. A T/C mutation at the position of the 1 326 bp of the PRL gene was found by DNA sequencing, and resulted in 3 genotypes （AA,AB and BB）. The frequencies of genotype BB and allele B were the highest. The least square analysis showed that the BB ducks had higher egg weight at the age of 30 weeks than AB ducks （P〈0.01）, the proportion of double-yolk of the AB ducks was higher than that of BB ducks （P〈0.05）, but there was not significant difference in egg number, the longest days of continual laying and the body weight at first laying egg among 3 genotypes.