菊粉体外培养猪鲜粪对其微生物数量的影响

Effect of Inulin on Microflora of Culture Medium with Fresh Feces of Growing Pigs

通过体外培养法探讨菊粉对猪鲜粪培养物pH和其中微生物数量的影响。试验设计0、1、2、4、6和8 g/L6个菊粉水平分别进行体外厌氧培养生长猪鲜粪,24 h后测定培养基pH、大肠杆菌、乳酸杆菌、双歧杆菌和总细菌数量的变化。结果表明,随着菊粉添加水平的增加,培养物pH和大肠杆菌数直线下降(P<0.001),而乳酸杆菌、双歧杆菌以及总细菌数则直线上升(P<0.01或P=0.059)。提示菊粉可促进猪鲜粪培养物中乳酸杆菌、双歧杆菌的增殖,抑制大肠杆菌的滋长,从而使总细菌数增加,pH下降,并具有剂量应答关系。

In order to determine the ferment properties and bifidogenic action of inulin, an in vitro fermentation experiment was conducted to anaerobically incubate fresh feces of growing pigs with inulin （0, 1, 2, 4, 6, 8 g/L, respectively） for 24 h at 37℃, and then determine pH and the populations of Escherichia coli, Lactobacillus, Bifidobacterium and total bacteria in the culture medium. The results showed that pH and the number of Escherichia coli linearly decreased （P〈 0. 001） ,and the number of Lactobacillus, Bifidobacterium and total bacteria in the culture medium linearly increased （P〈0.01 or P=0. 059）. It was concluded that there were dosage-response relationships between inulin additive level and bacteria （Escherichia coli, Lactobacillus, Bifidobacterium and total bacteria） amount or pH in the culture medium.