获得性免疫缺陷综合征患者白假丝酵母分离株基因型及耐药性分析

Genotyping and antifungal susceptibility of Candida albicans isolates from AIDS patients

目的研究获得性免疫缺陷综合征(AIDS)患者白假丝酵母分离株的基因型及耐药性。方法对分离自上海市公共卫生中心AIDS住院患者的40株白假丝酵母,应用微卫星核心序列引物M13进行聚合酶链反应(PCR)指纹分型。用微量稀释法分析白假丝酵母抗真菌药物敏感性。结果PCR指纹分型将所有白假丝酵母分离株分为A、B、C、D 4种基因型,其中A型14株(35.0%),B型16株(40.0%),C型9株(22.5%),D型1株(2.5%)。白假丝酵母对抗真菌药物的耐药率为:两性霉素B 2.5%,氟康唑22.5%,伊曲康唑15.0%,氟胞嘧啶20.0%,;不同基因型白假丝酵母菌株间耐药性差异无统计学意义。结论上海市AIDS住院患者分离的白假丝酵母主要由3种克隆组成,但无明显优势流行株。

Objective To investigate the genotypic profiles and antifungal susceptibility of Candida albicans isolates from patients with acquired immune deficiency syndrome （AIDS）. Methods A total of 40 isolates obtained from AIDS patients residented in Shanghai public health clinical center were screened for genotypic profiles using polymerase chain reaction （ PCR ） fingerprinting, based on the detection of DNA polymorphisms between minisatellite-specific sequences with the primer M13. Microdilution method was carried out for antifungal susceptibility test. Results PCR fingerprinting indicated 4 different genotypes among all isolates：genotype A strains 14 （35.0%）, genotype B strains 16 （40.0%） ,genotype C strains 9（22.5% ） ,genotype D strains 1 （2.5%）. The results of antifungal susceptibility test of Candida albicans isolates from patients with AIDS showed that amphotericin B was 2.5%, fluconazo was 22.5%, itraconazole was 15.0% and flucytosine was 20.0%. There was no significant difference of antifungal susceptibility between different genotypic strains. Conclusions Candida albicans isolates from （AIDS） patient in Shanghai primarily consist of 3 different genotypes, but there is no predominantly epidemic clone.