甘肃汉族人群白细胞介素1α启动子(-889C/T)基因型与冠心病的关系

The correlation between genotypes at the position-889C/T in the promoter of interleukin-1 alpha and coronary heart disease in Gansu province Han population

目的探讨白细胞介素-1α（IL-1α）启动子-889C/T基因型与冠心病（CHD）的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测98例CHD和131名健康者IL-1α-889C/T位点基因型。结果IL-1α-889位点在该人群中的基因型频率分布CC为183/229（79.9%）,CT为45/229（19.70%）,TT为1/229（0.04%）,等位基因C为411/458（89.70%）,T为47/458（10.30%）。CHD组CT基因型与对照组比较差异有统计学意义（P〈0.01）。心肌梗死组CT型与对照组比较差异有统计学意义（P〈0.01）,而心绞痛组中CT型与对照组比较差异无统计学意义（P〉0.05）,CHD组中CT型基因患心肌梗死的危险度是患心绞痛的2.02倍[比值比（OR）=2.02,95%可信区间（CI）：1.05-3.89,P〈0.05]。结论IL-1α启动子-889C/T位点存在基因多态性,T等位基因可能是甘肃汉族人群心肌梗死的易感基因。

Objective To investigate the correlation between the different genotypes at the position - 889C/T in the promoter of interleukin-1α（ IL-1 α） and the severity of coronary heart disease （CHD）. Methods The genotype of IL-1 α （-889C/T） from 98 patients with CHD and 131 healthy controls were analyzed by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP）. Results The frequence of genotype CC in Gansu province Han population were 183/229 （79.9%）, CT 45/229 （ 19.7% ） and TF 1/229 （0.04%）. The frequence of allele C was 411/458 （89.7%）, T 47/458 （ 10.3% ）. A significant increase was found for the IL-1 α genotype CT in CHD patients compared with controls （ P 〈 0.01 ）. There was significant difference in genotype CT between myocardial infarction group and controls （ P 〈 0.01 ）. But there was no significant difference in genotype CT between angina pectoris group and controls （ P 〉 0.05 ）. The relative risk of myocardial infarction group with genotype CT was 2.02 times to angina pectoris group （ odds ratio [ OR] = 2.02, 95% confidence interval [ CI] ： 1.05-3.89, P 〈 0.05）. Conclusions The polymorphism of genotypes C/T at the position -889 in the promoter of IL-1α is present and T allele may be a major risk factor for myocardial infarction patients in Gansu Han population.