Nocardia细胞的破碎方法及其IR光谱分析

Fragmentation Methods of Nocardia Cell and Its IR Spectral Analysis

研究了超声波法和自溶法对Nocardia的破碎情况．试验结果表明，这两种破碎方法均能使Nocardia得到较好的破碎，但是超声波法周期短，而自溶法周期长；全程破碎时间和输出功率是超声波法破碎的主要影响因素，采用短时多次的破碎方式和质量浓度为1．5g／L的细胞悬浮液有利于细胞的破碎．在Nocardia的自溶法破碎中，自溶时间是主要影响因素，NaCl质量分数为1％，温度为50℃利于细胞的自溶，pH值在7～9之间时，Nocardia内部各种酶的协同作用比较高．红外光谱分析结果表明，与Nocardia原菌相比，超声波法破碎和自溶法破碎后制得的Nocardia细胞壁所含有的基团类别基本相同．

The fragmentation of Nocardia was investigated by ultrasonic method and cell autolysis, and both could fragment well the microbe. But, the latter requires longer time than the former. The total time required and power output are the key influencing factors of ultrasonic method, and frequent short-time fragmenting steps and 1.5 g/L bacterium density are beneficial to cell fragmentation. In the cell autolysis process, time is the key factor, and that the NaCI density is 1% and temperature at 50 ℃ is beneficial to cell autolysis in which the collaborative effect of all enzymes in Nocardia is better when pH value is 7- 9. The result of IR spectral analysis indicated that the categories of radicals found in cell walls after either ultrasonic method or cell autolysis are almost the same.