新疆沙棘叶总黄酮的微波辅助提取和鉴别

Extration of Flavonoids from Sea-buckthom Leaves by Microwave-assisted Extraction Technique and Its Identification in Xinjiang

在微波辅助下,以乙醇为萃取剂,研究了新疆伊犁地区沙棘叶中总黄酮提取的工艺条件,采用单因素试验、正交试验确定了最佳工艺参数为:辐射时间3 min,固液比1∶20,乙醇浓度60%,微波火力为60%,溶液pH值为10,此时沙棘叶总黄酮提取率最高为2.02%,通过黄酮类化合物颜色反应的初步鉴定,确定其中含有黄酮及黄酮醇类等成分。

The experiment for microwave--assisted extracting total flavonoids from sea- buckthorn leaf of Hi in Xinjiang is carried out in this paper. The influences of various factors on extraction efficiencies were investigated across odd factorial experiments and orthogonal test. The optimum processing conditions are radiation time of 4min, solid - liquid ratio of 1：20, ethanol concentration is 60%, amount of power of 540, pH value of 10, now maximal extraction rate is 2.02%, flavone and flavonol were identified by initial color authentication in flavonoids.