黄芩甙对SAP大鼠多脏器细胞NF-κB表达的影响

Influence of Baicalin and Octreotide on NF-κB Expression in Multiple Organ Cells of Rats with Severe Acute Pancreatitis

目的:探讨黄芩甙对重症急性胰腺炎(SAP)大鼠肝、肾、肺的保护机制。方法:改良Aho法建立SAP大鼠模型并随机分为模型组、黄芩甙和奥曲肽治疗组,假手术组。造模后3h、6h、12h观察各组生存率、肝、肾、肺的病理变化及NF-κB表达水平。结果:治疗组12h生存率高于模型组(P<0.05),各脏器病理改变均不同程度减轻,肝、肾组织NF-κB含量及病理评分均小于模型组(P<0.05/P<0.01),其中黄芩甙治疗组12h点优于奥曲肽治疗组(P<0.05/P<0.01)。结论:黄芩甙和奥曲肽对SAP模型大鼠多脏器均有保护作用,并能有效地降低12h死亡率。

Objective To observe the influence of Baicalin and Octreotide on multiple organs （liver, kidney and lung）of rat with severe acute p ancreatitis and to evaluate the protecting mechanism Methods The modified Aho＇ s methods was adopted to prepare SAP rat models. All rats were randomly divided into the model group, Baicalin treatment group, Octreotide treatment group and the sham operation group. The survival rates of all groups, gross pathological changes of multiple organs and expression levels of NF- κB protein were observed at 3 h,6 h and 12 h after operation. Results The survival rates of the treatment groups were higher than that of the model group（ P 〈 0.05）. The pathological changes of multiple organs of the treatment groups were alleviated to different degrees. The NF - κB protein expression levels in the liver and kidney of treatment groups were lower than that of the model group （ P 〈 0.05 or P 〈 0.01 ）. The NF - κB level of Baicalin treatment group was lower than that of the Octreotide treatment group at 12 h （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Both Baicalin and Octreotide have obvious protecting effects on multiple organ injuries in SAP with the mechanism associated to inhibiting the NF - κB expression of the liver and kidney, Baicalin has a protecting effect similar to Octreotide.