生殖器疱疹实验诊断方法的临床应用探讨

Study on laboratory diagnosis assays of genital herpes infection

目的探讨生殖器疱疹实验诊断方法的临床应用价值。方法采用细胞培养法对82例临床诊断为典型生殖器疱疹的患者进行人类单纯疱疹病毒(HSV)分离培养,并同时进行聚合酶链反应(PCR)检查。结果两种方法的HSV检出率分别为58.5%和92.6%;培养法作为金标准特异性高,对早期典型水疱检出率高达89.7%;PCR与培养法相比敏感性高,尤其对糜烂或溃疡标本阳性检出率明显高于培养法。结论培养法适于早期检测,用于生殖器疱疹诊断特异性高;PCR操作具有简便、快速及敏感性高等特点,可用于不同皮损的检测。

Objective To explore the laboratory diagnosis of genital herpes infection. Methods Herpes simplex virus （HSV） was isolated in 82 patients with typical genital herpes by Vero cell method, and was detected by polymerase chain reaction （PCR） at the same time. Results The detection rate of cell culture and PCR was 58.5% and 92.6% re- spectively. The cell culture was highly specific with the detection rate of 89.7% for early typical vesicle. Compared with cell culture, PCR was more sensitive, especially for specimens soecunebs from erosion or ulcer. Conclusions The cell culture method is applicable for early lesion detection, and ishighly specific for the diagnosis of genital herpes infection. The advantage of PCR is simple rapid and sensitive, so it can he used to detect lesions from different skin changes.