汉族健康人群KIR分布对异基因造血干细胞移植供者选择的影响

The distribution of KIR gene in Chinese Han population influence donor selection of allogeneic stem cell transplantation

目的探讨杀伤细胞免疫球蛋白样受体(KIR)在中国人群中的分布规律及其对异基因造血干细胞移植供者选择的影响。方法采用序列特异性引物PCR(PCR-SSP)分型技术检测了79例汉族人群中KIR的基因型分布、74例异基因造血干细胞移植供受者对KIR及HLA基因型。结果KIR2DL1的基因分布频率为100%,KIR2DL2为20%,KIR2DL3为100%,KIR3DL1为94.81%。95.9%的供者携带与异基因造血干细胞移植关系密切的三组KIR受体。结论汉族人群具有独特的KIR分布规律,KIR2DL1、KIR3DL1是在中国人群异基因造血干细胞移植中起主要作用的KIR受体。

[ Objective ] To investigate the genotype distribution of killer cell immunoglobulin-like receptor （KIR） gene in Chinese Han population and the impact of distribution on donor selection of hematopoietic stem cell transplantation. [ Methods] The distribution of KIR in 79 healthy people, the donor KIR and patient HLA genotypes of allogeneic stem cell transplantation were analyzed by sequence specific primer-polymerase chain reaction, [ Results ] The gene frequencies of KIR2DL1 were 100%, KIR2DL2 were 20%, KIR2DL3 were 100%, KIR3DL1 were 94.81%. 95.9% of aUogeneic do- nors could be characterized as having an inhibitory KIR for each of the 3 known class I ligands. [ Conclusion ] Chinese Han population is unique in the distribution of KIR genotypes and putative KIR haplotypes compared with other ethnic groups. KIR2DL1 and KIR3DL1 play an important role in donor selection of Chinese stem cell transplantation.