高血压患者AT1R基因多态性与药物降压关系

Relationship between gene polymorphism of AT1R gene of hypertension and medication-reduced blood pressure

目的分析原发性高血压患者血管紧张素Ⅱ受体Ⅰ(angiotensinⅡreceptorⅠgene,AT1R)基因(A1166)多态性及环境因素与苯那普利降压疗效的关系。方法对来自安徽西部的原发性高血压患者进行了服用苯那普利前后的血压随访观察,同时采用多聚合酶链反应-限制性片段长度多态性(PCR-PFLP)法测定了患者(AT1R)基因(A1166C)多态性分布频率。结果其中A等位基因频率为96%,C等位基因频率为8%,且符合Hardy-Weinberg平衡检测。经χ2检验(χ2=0.31,P>0.05)符合H-W平衡。Logistic回归分析结果显示,不同AT1R基因型的患者服药后的血压达标率在Ⅰ期高血压人群中差异有统计学意义。结论本次研究在国内首次测得AT1R(A1166C)的CC型突变;AT1R的CC1166等位基因可能对ACEI类降压药物具有受体敏感性。

Objective To analyze the correlation between polymorphism of the angiotensin Ⅱ receptor Ⅰ gene（AT1R, A1166C）, environment factors, and the treatment effect of antihypertensive drug-Bnenapril. Methods To observe the blood pressure of the subjects before and after taking Bnenapril in essential hypertension patients in west of Anhui. Polymerase chain reaction（PCR） combined with restriction fragment length polymorphism（RFLP） were used to detect genetype frequence and polymorphism for AT1R. Results The study revealed that the frequency of allele A and C accounted for 96 % and 4% in the total populations. Through chi-square test, it met with H - W balance（ χ^2 = 0.31, P 〉 0.05 ）. The achieved target rates of Ⅰ period hypertensives showed the difference between AA and AC/CC groups after taking Benapril. Conclusion This was the first time in China to detect the CC of AT1R（A1166C） gene polyrnorphism. The C allele of AT1R gene might be receptor sensitivity to the ACEI drugs.