杉木花粉母细胞减数分裂的细胞学特性及异常现象的观察

Cell Biological Characteristics and Abnormal Behavior during the Meiosis of Pollen Mother Cells in Cunninghamia lanceolata

用2种荧光染料(CMA、DAPI)对3个杉木无性系花粉母细胞减数分裂的染色体进行荧光染色,发现在减数分裂的花粉母细胞的各个时期,染色质或染色体上有CMA荧光亮点出现,而无DAPI荧光亮点。在杉木花粉母细胞减数分裂过程中观察到一些异常细胞学行为,即单价体和多价体、染色体桥、滞后染色体、不均等分离、微核等异常现象。对杉木花粉的生活力差异与杉木小孢子形成过程中减数分裂异常现象之间的关系进行了讨论。

The chromosomal biological characteristics of 3 different clones were analysed with CMA and DAPI（fluorescent dye） during the meiosis of pollen mother cell in Cunninghamia lanceolata. Some fluorescent bands were detected in the chromosomes with CMA, but none with DAPI. In addition, some abnormal cytology behaviors, such as＇ univalence, multivalence, chromosome bridges, lagging chromosomes, non-equivalent division, and micro-nucleus, etc. were also observed in the meiosis of pollen mother cell. At last, the relation between abnormal phenomena in meiosis of pollen mother cell and pollen sterility of C. lanceolata was discussed from cytogenetis levels.