绣线菊丛枝病病原的分子鉴定

Molecular Identification of the Phytoplasma Associated with Spiraea Witches' Broom

用植原体16S rRNA基因的通用引物,对表现黄化、丛枝、顶枯等症状的绣线菊DNA进行PCR扩增,得到了约1.2kb的特异性片段。测序结果证明其病原为植原体。RFLP和序列分析表明:该分离物属于翠菊黄化组的16SrⅠ-B亚组。与GenBank中其他植原体分离物序列比较,发现该分离物与16SrⅠ-B亚组中的西方翠菊黄化植原体(SAY)同源性高达99.6%。

A specific fragment of ca. 1.2 kb in length was amplified with PCR from spiraea plants showing typical phytoplasma symptom of yellowing, witches＇ broom and dieback. The fragment was sequenced and subjected to RFLP and phylogenetic analysis. RFLP analysis showed that the phytoplasma associated with spiraea witches＇ broom belonged to 16Sr I-B subgroup of aster yellows phytoplasma group. This phytoplasma shared the highest homology of 99.6% with severe aster yellows （SAY） in subgroup 16Sr I-B.