超抗原活化淋巴细胞对喉癌Hep-2细胞系的抑制作用

Inhibition of superantigen-activated lymphocytes on the growth of laryngeal carcinoma Hep-2 cells

目的观察超抗原活化淋巴细胞后对喉癌细胞的抑制作用。方法金黄色葡萄球菌肠毒素A与人淋巴细胞混合培养24h,激活淋巴细胞,再加入人喉鳞状细胞癌Hep-2细胞混合培养72h,通过MTT法观察喉癌细胞的抑制情况。结果①实验组金黄色葡萄球菌肠毒素A激活淋巴细胞后对人喉鳞癌Hep-2细胞的生长有明显的抑制作用,抑制率是42.68%,高于对照组4.82%(P<0.01)。②抑制作用呈现时间的依赖性。③金黄色葡萄球菌肠毒素A在不同浓度时对喉癌细胞的生长均有抑制作用,特别在浓度是1μg/ml、72h培养时抑制率最高,达82.83%。结论超抗原活化淋巴细胞后明显抑制喉癌细胞的生长。

Objective To study the inhibitory effects of superantigen - etivated lymphocytes on the growth of laryngeal carcinoma. Methods Staphylococcal enterotoxin A and human lymphocytes were mixed and cultured for 24 hours. The activated lymphocytes were subsequently co - cultured with human laryngeal squamose cell carcinoma Hep - 2 cells for 24 hours, Inhibition of the growth of laryngeal carcinoma cells was measured by MTF assays. Results ①Lymphocytes activated by Staphylococcal enterotoxin A inhibietd the proliferation of human laryngeal squamose cell carcinoma Hep - 2 cells with an inhibitory rate 42, 68% in the experimental group, significantly higher than that in the contral group （4. 82% ,P 〈0. 01 ）, ②The inhibitory effect was time - dependence, ③Staphylococcal enterotoxin A at different concentration showed inhibitory effects and the maximal inhibitory rate of 82. 83% was observed at the concentration was 1 μg/ml for 72 h. Conclusion Superantigen significantly inhibits the growth of laryngeal carcinoma cell via activating lymphocytes.