大鼠鞘内腺苷同类物CHA抗神经病理性疼痛作用

Intrathecal analgesic effects of adenosine analog CHA in rats with neuropathic pain

目的探讨大鼠鞘内注射腺苷同类物CHA(Cyclohexyladenosine)抗神经病理性疼痛作用。方法雄性SD大鼠,鞘内留置PE-10导管,一周后制作大鼠脊神经结扎神经病理性疼痛模型(SNL)。将30只SNL大鼠随机分成5组(n=6):对照组(生理盐水)、CHA组(CHA0.1,0.5,1.0nmol)、拮抗组(CHA1.0nmol+格列本脲2.0μg)。测定各组鞘内给药前、给药后10,20,30,40,50,60min各时点的大鼠机械撤足阈值,计算对应时点的最大效应百分比(%MPE),评价抗神经病理性疼痛效果。结果CHA0.5及1.0nmol组抗神经病理性疼痛最大效应百分比均明显高于生理盐水组(P<0.05)。CHA1.0nmol+格列本脲2.0μg组抗神经病理性疼痛最大效应百分比明显低于CHA1.0nmol组(P<0.05)。结论鞘内注射CHA具有明显的抗神经病理性疼痛作用,鞘内预注格列本脲(三磷酸腺苷敏感型K+通道阻滞剂)能拮抗CHA抗神经病理性疼痛作用。

Objective To investigate the analgesic effects of adenosine analog CHA （Cyclohexyladenosine） administered into subarachnoid space in rats with neuropathic pain. Methods Male SD rats were chronically implanted with lumbar intrathecal catheters. One week later, the left L5 and L6 spinal nerve roots were ligated, to ereat spinal nerve ligation neuropathie pain models （SNL）. Thirty SD rats with lumbar intratheeal catheters and SNL model were randomly divided into 5 groups with 6 rats each：control group（saline）, CttA 0.1 nmol, CHA 0.5 mnol, CHA 1.0 nmol and antagonist group（ CHA 1.0 nmol and glibenclamide 2. 0 μg）. Mechanical withdrawal threshold at the time points of before, 10, 20,30,40,50 and 60 rain after intratheeal administration was evaluated. The maximum possible effect（ % MPE） was caleurated and the anti - neuropathie pain effects was estimated. Results CHA 0. 5 nmol group and 1.0 nmol group produced significant anti - neuropathie pain effects than the saline group （ P 〈 0. 05 ）. CHA 1.0 nmol and glibenclamide 2. 0 μg group produced less anti - neuropathic pain effects than CHA 1.0 nmol group（P 〈 0.05 ）. Conclusion Intrathecally administered CHA produees significant anti - neuropathic pain effects. The effect of CHA can be blocked by glibenelamide （ ATP sensitivity K ^＋ehannet antagonist）.